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IL-13Ra1 Polyclonal antibody

IL-13Ra1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27916-1-AP

Synonyms

IL13RA1, IL 13R subunit alpha 1, IL 13R alpha 1, IL 13 receptor subunit alpha 1, CT19

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-13Rα1 Polyclonal antibody (27916-1-AP)

    WB analysis using 27916-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27916-1-AP (IL-13RA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHT-29 cells, Jurkat cells, Raji cells, mouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27916-1-AP targets IL-13Ra1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-13Ra1 fusion protein Ag25542
Full Name interleukin 13 receptor, alpha 1
Calculated Molecular Weight 49 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC009960
Gene Symbol IL-13Rα1
Gene ID (NCBI) 3597
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-13 is an immunoregulatory cytokine secreted predominantly by activated T-helper 2 (Th2) cells and performs multiple functions through a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2 (PMID: 12704343). IL-13Rα1 is a 65- to 70-kd glycosylated transmembrane protein (PMID: 10856136; 9723655). IL-13Rα1 is expressed on a wide range of cells including B cells, monocytes, macrophages, basophils, mast cells, and endothelial cells (PMID: 10856136; 31866859). IL-13 binding to the IL-4Rα/IL-13Rα1 complex leads to activation of JAK1, JAK2, and TYK2 and subsequent phosphorylation of STAT6 and STAT3 (PMID: 31866859).

Protocols

Product Specific Protocols
WB protocol for IL-13Ra1 antibody 27916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Ciara (Verified Customer) (01-30-2024)

Positive staining in N2a cells

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: Neuro-2a mouse neuroblastoma cell line