Anti-Mouse IL-13 Rabbit Recombinant Antibody

IL-13 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

2C14

Cat no : 98003-1-RR

Synonyms

Il13, IL-13, interleukin 13, Interleukin-13, 2C14, Il 13

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Validation Data Gallery

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  FC experiment of C57BL/6 Th2-polarized splenocytes using 98003-1-RR

  1x10^6 unstimulated or PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes were intracellularly stained with 0.25 ug Anti-Mouse IL-13 Rabbit Recombinant Antibody (98003-1-RR, Clone:2C14) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98003-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98003-1-RR against Mouse IL-13 were performed. The affinity constant is 0.787 nM.

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Tested Applications

• Positive FC (Intra) detected in: PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes
• Positive FC detected in: PMA and ionomycin stimulated C57BL/6 Th2-polarized splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98003-1-RR targets IL-13 in FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 13
• Calculated Molecular Weight: 14 kDa
• GenBank Accession Number: NM_008355
• Gene Symbol: IL-13
• Gene ID (NCBI): 16163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Interleukin 13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. IL-13 up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. IL-13 inhibits the production of a series of cytokines like IL-1, IL-6, TNF-alpha, and IL-8 by activated human monocytes. IL-13 induces IFN-gamma production by NK cells. IL-13 is thought to be important cytokine in the pathogenesis of asthma, and more recently has been shown to play a pivotal role in a number of fibrotic diseases including hepatic and pulmonary fibrosis, and nodular sclerosing Hodgkin's disease.