IL-13 Recombinant antibody

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  WB analysis of EL-4 using 83935-1-RR

  Untreated EL-4 and EL-4 (treated with 50ng/mL PMA and Ionomycin 500ng/mL for 6h, replaced the culture medium with Brefeldin A for 18 hours) cells were subjected to SDS PAGE followed by western blot with 83935-1-RR (IL-13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83935-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83935-1-RR against Rat IL-13 were performed. The affinity constant is 0.419 nM.

IL-13 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83935-1-RR

Clone No.240771B9

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Il13, interleukin 13, 240771B9

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Tested Applications

• Positive WB detected in: PMA, Ionomycin and Brefeldin A treated EL-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83935-1-RR targets IL-13 in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 13
• Calculated Molecular Weight: 14kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: NM_053828.1
• Gene Symbol: Il13
• Gene ID (NCBI): 116553
• RRID: AB_3671517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P42203
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rat IL-13 is a kind of cytokines, which plays important roles in allergic inflammation and immune response to parasite infection. (PubMed:9366558). Rat IL-13 displays the capacity to antagonize Th1-driven proinflammatory immune response and downregulates synthesis of many proinflammatory cytokines including IL1, IL6, IL10, IL12 and TNF-alpha through a mechanism that partially involves suppression of NF-kappa-B (PubMed:9366558).