IL-12/IL-23 p40 Polyclonal antibody

IL-12/IL-23 p40 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 30956-1-AP

Synonyms

IL12B, IL 23, IL 12B, IL 12 subunit p40, IL 12 P40

---

Validation Data Gallery

• 

  WB analysis using 30956-1-AP

  Human IL-12B/p40, IL-12/p70, IL-12A/p35 recombinant protein, were subjected to SDS PAGE followed by western blot with 30956-1-AP (IL-12B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30956-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30956-1-AP (IL-12B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of Jurkat using 30956-1-AP

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: Recombinant protein, HepG2 cells, Jurkat cells
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30956-1-AP targets IL-12/IL-23 p40 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
• Calculated Molecular Weight: 328 aa, 37 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC067498
• Gene Symbol: IL12B
• Gene ID (NCBI): 3593
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).