IL-12 p70 Recombinant antibody

IL-12 p70 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230037B7

Cat no : 82878-2-RR

Synonyms

Il 12b, Il 12p40, IL-12 P35, IL-12 p70, Il12b, Il12p40, interleukin 12b, p40

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Validation Data Gallery

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  WB analysis of Recombinant protein using 82878-2-RR

  Recombinant protein Eg0742 (IL-12 p70) were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82878-2-RR

  Recombinant protein Eg0742 (IL-12 p70) and Recombinant protein Eg1821 (IL-12A p35)were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82878-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82878-2-RR against Mouse IL-12 p70 were performed. The affinity constant is 7.37 nM.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82878-2-RR is IL-12 p70 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interleukin 12b
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• Gene Symbol: Il12b
• Gene ID (NCBI): 16160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.