IL-12 p70 Recombinant antibody

IL-12 p70 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230295F4

Cat no : 83094-1-RR

Synonyms

IL-12, Interleukin 12, IL12, IL 12

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Validation Data Gallery

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  WB analysis using 83094-1-RR

  Recombinant protein IL-12 p70(Eg0575) was subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83094-1-RR

  Recombinant protein IL-12B p40(Eg0431) were subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83094-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83094-1-RR against Human IL-12 p70 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83094-1-RR targets IL-12 p70 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: IL-12 p70
• Observed Molecular Weight: 70 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells.