Recombinant protein IL-12 p70(Eg0575) was subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Recombinant protein IL-12 p70(Eg0575) was subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 83094-1-RR
Recombinant protein IL-12B p40(Eg0431) were subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Recombinant protein IL-12B p40(Eg0431) were subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Affinity and Kinetic Characterization of 83094-1-RR
Biolayer interferometry (BLl) kinetic assays of 83094-1-RR against Human IL-12 p70 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
IL-12 is a heterodimeric cytokine composed of two glycosylated and disulfide-linked subunits (p40 cysteine-linked to p35). IL-12 is a potent regulator of cell mediated immune responses and it induces IFN-gamma production by NK and T cells. It is produced by activated monocytes/macrophage cells, B lymphocytes, and connective tissue type mast cells.
Recombinant protein IL-12 p70(Eg0575) was subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 83094-1-RR
Recombinant protein IL-12B p40(Eg0431) were subjected to SDS PAGE followed by western blot with 83094-1-RR (IL-12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
AFFINITY Figures
Affinity and Kinetic Characterization of 83094-1-RR
Biolayer interferometry (BLl) kinetic assays of 83094-1-RR against Human IL-12 p70 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IL-12 p70 Recombinant antibody
Catalog Number
83094-1-RR
Citations
-
Dilutions
WB : 1:2000-1:10000
Applications
WB, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
