Anti-Mouse IL-12/IL-23 p40 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of RAW 264.7 using 98004-1-PBS

  1X10^6 unstimulated or IFNγ and LPS treated (in the presence of protein transport inhibitors) RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-12/23 p40 (98004-1-PBS, Clone: 230037C9) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:700. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of RAW 264.7 using 98004-1-RR (same clone as 98004-1-PBS)

  1X10^6 unstimulated or IFNγ and LPS treated (in the presence of protein transport inhibitors) RAW 264.7 cells were intracellularly stained with 0.125 ug Anti-Mouse IL-12/IL-23 p40 (98004-1-RR, Clone: 230037C9) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:700. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98004-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98004-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98004-1-RR against Mouse IL-12/23 p40 were performed. The affinity constant is 4.18 nM.

IL-12/IL-23 p40 {ptg:Brand} Recombinant Antibody for FC (Intra)

Cat No. 98004-1-PBS

Clone No.230037C9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Il12b, Il-12b, IL-12 p40, Il 12p40, Il 12b

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Tested Applications

• Positive FC (Intra) detected in: IFNγ and LPS treated RAW 264.7 cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98004-1-PBS targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: IL-12/IL-23 p40
• Calculated Molecular Weight: 70 kDa
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P43432
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C. Stable for one year after shipment.

Background Information

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).