IL-11 Monoclonal antibody

IL-11 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F3A12

Cat no : 68135-1-Ig

Synonyms

IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor

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Validation Data Gallery

• 

  WB analysis using 68135-1-Ig

  Saos-2 cell supernatant (cells stimulated with 500ng/mL HGF for 4 days) were subjected to SDS PAGE followed by western blot with 68135-1-Ig (IL-11 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Saos-2 supernatant cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68135-1-Ig targets IL-11 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=1.80 x 10^-9M
  K_Off=2.20 x 10^-6M
  K_On=1.22 x 10^3M
• Immunogen: IL-11 fusion protein Ag11199
• Full Name: interleukin 11
• Calculated Molecular Weight: 199 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC012506
• Gene Symbol: IL-11
• Gene ID (NCBI): 3589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-11 (IL-11) is a member of the glycoprotein-130 (GP-130) cytokines that utilizes the GP-130 signaling pathway shared by other cytokines of the same family. The most well-known role of IL-11 is in hematopoiesis because it promotes megakaryocytopoiesis, erythropoiesis, and thrombopoiesis. In recognition of these properties, human IL-11 recombinant protein (rhIL-11) is used clinically for treating chemotherapy-induced thrombocytopenia. IL-11 has extensive immunomodulatory effects, both anti- and proinflammatory.