IL-10RA Polyclonal antibody, PBS Only

IL-10RA Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13356-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 13356-1-AP (same clone as 13356-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL-10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13356-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 13356-1-AP (same clone as 13356-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13356-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 13356-1-AP (same clone as 13356-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13356-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 13356-1-AP (same clone as 13356-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13356-1-PBS in a different storage buffer formulation.

---

Product Information

13356-1-PBS targets IL-10RA in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-10RA fusion protein Ag4156
• Full Name: interleukin 10 receptor, alpha
• Calculated Molecular Weight: 578 aa, 63 kDa
• Observed Molecular Weight: 70-72 kDa, 90-95 kDa
• GenBank Accession Number: BC028082
• Gene Symbol: IL-10RA
• Gene ID (NCBI): 3587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RA, also known as IL-10R1 or CD210, is expressed on most hematopoietic cells at a basal level but is upregulated by various cells upon activation (PMID: 24507158). IL-10RA serves as the ligand-binding subunit of the receptor complex. Upon binding of IL-10 to the IL-10R complex, the kinases JAK1 and TYK2 are activated and catalyze the phosphorylation of IL-10RA at specific intracellular tyrosine residues, leading to the recruitment and subsequent phosphorylation of STAT3 (PMID: 11244051; 10433356).