IL-1 Beta Recombinant antibody, PBS Only (Capture)

IL-1 Beta Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3I9

Cat no : 82696-6-PBS

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

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Validation Data Gallery

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  WB analysis of THP-1 using 82696-6-RR (same clone as 82696-6-PBS)

  LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 82696-6-RR (IL1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82696-6-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP80001-1

  Cytometric bead array standard curve of MP80001-1, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-6-PBS. Detection antibody: 65276-1-PBS. Standard:HZ-1164. Range: 6.25-100 ng/mL.

• 

  Cytometric bead array standard curve of MP80001-2

  Cytometric bead array standard curve of MP80001-2, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-6-PBS. Detection antibody: 68767-1-PBS. Standard:HZ-1164. Range:3.125-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 82696-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82696-6-RR against Human IL-1 Beta were performed. The affinity constant is 202 nM.

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Product Information

82696-6-PBS targets IL-1 Beta as part of a matched antibody pair:

MP80001-1: 82696-6-PBS capture and 65276-1-PBS detection (validated in Cytometric bead array)

MP80001-2: 82696-6-PBS capture and 68767-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 1, beta
• Calculated Molecular Weight: 269 aa, 31 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC008678
• Gene Symbol: IL1B
• Gene ID (NCBI): 3553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. IL-1 Beta(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions. Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form.