IL-1 alpha Polyclonal antibody

IL-1 alpha Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 28475-1-AP

Synonyms

IL1A, IL 1 alpha, Hematopoietin-1, IL-1,IL1, IL1 ALPHA

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Validation Data Gallery

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  WB analysis using 28475-1-AP

  Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 28475-1-AP (IL-1 alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

28475-1-AP targets IL-1 alpha in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-1 alpha fusion protein Ag29587
• Full Name: interleukin 1, alpha
• Calculated Molecular Weight: 271 aa, 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC013142
• Gene Symbol: IL-1 alpha
• Gene ID (NCBI): 3552
• RRID: AB_2918169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; ZeXieYin formula alleviates atherosclerosis by inhibiting the MAPK/NF-κB signaling pathway in APOE-/- mice to attenuate vascular inflammation and increase plaque stability; Authors - Rumin Huang
mouse	WB	Brain Res Bull; Integrated Analysis of circRNA- related ceRNA Network Targeting Neuroinflammation in Medial Temporal Lobe Epilepsy; Authors - Bingzheng Gong