IKZF4 Polyclonal antibody

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  WB analysis using 31094-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31094-1-AP (IKZF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IKZF4 Polyclonal Antibody for WB, ELISA

Cat No. 31094-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: K-562 cells, Raji cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31094-1-AP targets IKZF4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IKZF4 fusion protein Ag34767
• Full Name: IKAROS family zinc finger 4 (Eos)
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC160169
• Gene Symbol: IKZF4
• Gene ID (NCBI): 64375
• RRID: AB_3669852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2S9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKZF4 is a member of the Ikaros family transcription factors. The Ikaros family was originally designated to function in hematopoiesis, but it is only recently that studies have begun to show the relevance of this family in T helper cell differentiation. IKZF3 was preferentially expressed in TH17 cells and governs TH17 differentiation by silencing IL-2 expression. By investigating the dynamics of the TH17 regulatory network, IKZF4 was proposed by Yosef et al. as a composite in the negative regulatory module for TH17. (PMID: 24644282)