HELIOS Polyclonal antibody

HELIOS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13554-1-AP

Synonyms

IKZF2, ZNFN1A2, ZNF1A2, Zinc finger protein Helios, IKZF 2

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Validation Data Gallery

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  WB analysis of Jurkat using 13554-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13554-1-AP (HELIOS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13554-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13554-1-AP (HELIOS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 13554-1-AP

  IP result of anti-HELIOS (IP:13554-1-AP, 4ug; Detection:13554-1-AP 1:300) with Jurkat cells lysate 2400ug.

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  IHC staining of human lymphoma using 13554-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13554-1-AP (HELIOS antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lymphoma using 13554-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 13554-1-AP (IKZF2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 13554-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13554-1-AP (HELIOS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, human placenta tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13554-1-AP targets HELIOS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HELIOS fusion protein Ag4357
• Full Name: IKAROS family zinc finger 2 (Helios)
• Calculated Molecular Weight: 500 aa, 57 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC028936
• Gene Symbol: HELIOS
• Gene ID (NCBI): 22807
• RRID: AB_2124959
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ikaros DNA-binding proteins, characterized by two highly conserved zinc finger domains that mediate DNA (N-terminal domain) and protein binding (C-terminal domain), consists of 5 members and one of them is IKZF2 [PMID:1439790]. IKZF2's expression is associated with T cell activation and cellular division and is suggested a suitable marker to distinguish natural and induced Treg (PMID:21918685).

Publications

Species	Application	Title
mouse	IF	Immunohorizons; Clonal Bifurcation of Foxp3 Expression Visualized in Thymocytes and T Cells.; Authors - Bonnie Yen