IKZF1 Monoclonal antibody, PBS Only

IKZF1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, Rabbit, Pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D6B5

Cat no : 66966-1-PBS

Synonyms

DNA binding protein Ikaros, hIk 1, Hs.54452, IK1, IKAROS, IKZF1, LYF1, PRO0758, ZNFN1A1

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Validation Data Gallery

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  WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit spleen using 66966-1-Ig (same clone as 66966-1-PBS)

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig thymus using 66966-1-Ig (same clone as 66966-1-PBS)

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66966-1-Ig (same clone as 66966-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66966-1-Ig (IKZF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66966-1-Ig (same clone as 66966-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66966-1-Ig (IKZF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66966-1-PBS in a different storage buffer formulation.

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Product Information

66966-1-PBS targets IKZF1 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, Rabbit, Pig samples.

• Tested Reactivity: Human, mouse, Rabbit, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IKZF1 fusion protein Ag28637
• Full Name: IKAROS family zinc finger 1 (Ikaros)
• Calculated Molecular Weight: 477 aa, 53 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC018349
• Gene Symbol: IKZF1
• Gene ID (NCBI): 10320
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IKZF1, also known as DNA-binding protein Ikaros, Belongs to the Ikaros C2H2-type zinc-finger protein family. The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin. The C-terminal zinc-finger domain is required for dimerization. IKZF is abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. IKZF1 is localized in nucleus. IKZF1 as a transcription regulator of hematopoietic cell differentiation binds gamma-satellite DNA and plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. IKZF1 is a repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. IKZF1 exists some isofroms with MV 58KDa, 48KDa, 48KDa, 43KDa, 41KDa, 32KDa and 53KDa, respectively.