IK Polyclonal antibody

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  WB analysis using 31833-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31833-1-AP (IK antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 31833-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31833-1-AP (IK antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 31833-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using IK antibody (31833-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

IK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31833-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RED, Protein RER, Protein Red, IK factor, IK Cytokine

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, HepG2 cells, SH-SY5Y cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1500-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31833-1-AP targets IK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IK fusion protein Ag37095
• Full Name: IK cytokine, down-regulator of HLA II
• Calculated Molecular Weight: 557 aa, 66 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC013005
• Gene Symbol: IK
• Gene ID (NCBI): 3550
• RRID: AB_3670123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q13123
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IK, also known as the nuclear protein RED, is a down-regulator of human leukocyte antigen (HLA) II and interacts with the human SAC protein MAD1, which is required for the localization of the kinetochore of MAD1, for the progression of mitosis and for the activation of the SAC. IK is therefore an important protein for the correct progression of mitosis through the regulation of mitotic kinases and phosphatases. In addition, IK may be involved in pre-mRNA splicing, which may be a useful biomarker for new nuclear structures in cells.