IHCeasy MYLK Ready-To-Use IHC Kit

MYLK Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat no : KHC2496

Synonyms

MYLK,EC:2.7.11.18,Kinase-related protein,KRP,MLCK



Product Information

KHC2496 is a ready-to-use IHC kit for staining of MYLK. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy MYLK Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

MYLK(Myosin light chain kinase) is also named as MLCK, MLCK1, MYLK1 and belongs to the protein kinase superfamily. It is a key enzyme in muscle contraction. Its activation is sufficient to enhance paracellular permeability and is required for tight junction barrier regulation in response to Na+-nutrient cotransport, inflammatory cytokines, or pathogenic bacteria. The expression of smooth muscle MYLK is tissue-specific. Smooth muscle MYLK generates three different proteins, long MYLK, short MYLK, and telokin. The 130-kDa smooth muscle, or short, MYLK (130 kDa) is expressed in smooth muscles.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms MYLK,EC:2.7.11.18,Kinase-related protein,KRP,MLCK