IHCeasy FEN1 Ready-To-Use IHC Kit
FEN1 Ready-to-use reagent kit for IHC.
Cat no : KHC2256
Synonyms
Validation Data Gallery
Product Information
KHC2256 is a ready-to-use IHC kit for staining of FEN1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy FEN1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance. FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesi. FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms |