IHCeasy DSG1 Ready-To-Use IHC Kit
DSG1 Ready-to-use reagent kit for IHC.
Cat no : KHC0259
Synonyms
Cadherin family member 4, CDHF4, desmoglein 1, Desmosomal glycoprotein 1, DG1, DGI, DSG, DSG1, Pemphigus foliaceus antigen, PPKS1, SPPK1
Validation Data Gallery
Product Information
KHC0259 is a ready-to-use IHC kit for staining of DSG1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy DSG1 Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Rabbit Polyclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Rabbit |
| Reactivity | Human, Mouse |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein that is highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers. DSG1 mediates intercellular adhesion and have a crucial role in maintaining epidermal integrity and barrier function. It is also involved in epithelial cell differentiation. Mutations in the DSG1 gene can cause the autosomal dominant disorder striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies and metabolic wasting (SAM syndrome).
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | Cadherin family member 4, CDHF4, desmoglein 1, Desmosomal glycoprotein 1, DG1, DGI, DSG, DSG1, Pemphigus foliaceus antigen, PPKS1, SPPK1 |