IHCeasy CR1 Ready-To-Use IHC Kit

CR1 Ready-to-use reagent kit for IHC.

Reactivity

Human

Sample Type

FFPE tissue

Cat no : KHC0102

Synonyms

C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN



Product Information

KHC0102 is a ready-to-use IHC kit for staining of CR1. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy CR1 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Mouse Monoclonal
Secondary antibody type Polymer-HRP-Goat anti-Mouse
Reactivity Human

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

Complement receptor type 1 (CR1), also known as C3b/C4b receptor or CD35, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CR1 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhan cells and glomerular podocytes (PMID: 19004497). CR1 acts as a receptor for C3b and C4b and plays important roles in regulation of the complement cascade and clearance of immune complexes. A soluble form of CR1 (sCR1) has been found in plasma (PMID: 3156931). It has been reported that sCR1 is produced by a proteolytic cleavage in the C-terminal region of CR1 transmembrane domain (PMID: 9405292).

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms C3b/C4b receptor, C3BR, CD35, Complement receptor type 1, CR1, KN