IHC Prep & Detect Kit for Rabbit/Mouse Primary Antibody

Sample type

FFPE tissue

Assay type


Secondary antibody host species


Secondary antibody species specificity


Cat no : PK10019

Product Information

The PK10019 kit is designed for developing a complete IHC workflow on FFPE samples using primary antibodies raised in rabbits/mice. The kit contains all the basic reagents, starting from antigen retrieval buffer to mounting media, that would be required for IHC staining of tissue sections with rabbit/mouse primary antibodies. By simply optimizing the primary antibody concentration and a few operational parameters, a complete IHC workflow can be easily established using this kit.

* If you are planning to use a rabbit primary antibody for staining mouse samples, we recommend using our IHC Prep & Detect Kit for Rabbit Primary Antibody (Cat.NO. PK10017).


Part NO.ComponentSize
HC001Antigen Retrieval Buffer (50× Tris-EDTA)100 mL
HC002Washing Buffer (30×)120 mL
HC003Blocking Buffer5 mL
HC000Quenching Buffer (RTU)5 mL
HC004Primary Antibody Diluent (RTU)100 mL
HC005Polymer HRP-Goat anti-Rabbit/Mouse Secondary Antibody5 mL
HC006Chromogen Component A0.2 mL
HC007Chromogen Component B4 mL
HC008Signal Enhancer5 mL
HC009Counter Staining Reagent5 mL
HC0010Mounting Media5 mL

Size:  50-80 tests (may vary depending on tissue size).


All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.

Application Note

This kit is recommended for FFPE tissues and not for frozen sections.

This kit contains an antigen retrieval buffer based on Tris-EDTA by default, which in our experience is suitable for over 90% of targets. However, if a negative result is obtained using this buffer, we recommend trying alternative buffers listed below.

# PR30001 Sodium Citrate Antigen Retrieval Buffer 50x

# PR30014 Protease K Retrieval Buffer (A+B) (Heating is not required with this buffer)

Refer to user’s manual for instructions on developing an IHC workflow using this kit.

Cited in Article as

PK10019, IHC Prep & Detect Kit for Rabbit/Mouse Primary Antibody, Proteintech, IL, USA



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