Cat no : PK10019
The PK10019 kit is designed for developing a complete IHC workflow on FFPE samples using primary antibodies raised in rabbits/mice. The kit contains all the basic reagents, starting from antigen retrieval buffer to mounting media, that would be required for IHC staining of tissue sections with rabbit/mouse primary antibodies. By simply optimizing the primary antibody concentration and a few operational parameters, a complete IHC workflow can be easily established using this kit.
* If you are planning to use a rabbit primary antibody for staining mouse samples, we recommend using our IHC Prep & Detect Kit for Rabbit Primary Antibody (Cat.NO. PK10017).
|HC001||Antigen Retrieval Buffer (50× Tris-EDTA)||100 mL|
|HC002||Washing Buffer (30×)||120 mL|
|HC003||Blocking Buffer||5 mL|
|HC000||Quenching Buffer (RTU)||5 mL|
|HC004||Primary Antibody Diluent (RTU)||100 mL|
|HC005||Polymer HRP-Goat anti-Rabbit/Mouse Secondary Antibody||5 mL|
|HC006||Chromogen Component A||0.2 mL|
|HC007||Chromogen Component B||4 mL|
|HC008||Signal Enhancer||5 mL|
|HC009||Counter Staining Reagent||5 mL|
|HC0010||Mounting Media||5 mL|
Size: 50-80 tests (may vary depending on tissue size).
All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
This kit is recommended for FFPE tissues and not for frozen sections.
This kit contains an antigen retrieval buffer based on Tris-EDTA by default, which in our experience is suitable for over 90% of targets. However, if a negative result is obtained using this buffer, we recommend trying alternative buffers listed below.
# PR30001 Sodium Citrate Antigen Retrieval Buffer 50x
# PR30014 Protease K Retrieval Buffer (A+B) (Heating is not required with this buffer)
Refer to user’s manual for instructions on developing an IHC workflow using this kit.
Cited in Article as
PK10019, IHC Prep & Detect Kit for Rabbit/Mouse Primary Antibody, Proteintech, IL, USA
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