IGSF21 Polyclonal antibody

• Featured Product
• KD/KO Validated

IGSF21 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21465-1-AP

Synonyms

IGSF21

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Validation Data Gallery

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  WB analysis of mouse brain using 21465-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21465-1-AP (IGSF21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 21465-1-AP is IGSF21 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGSF21 fusion protein Ag15062
• Full Name: immunoglobin superfamily, member 21
• Calculated Molecular Weight: 467 aa, 52 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC007618
• Gene Symbol: IGSF21
• Gene ID (NCBI): 84966
• RRID: AB_10734429
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGSF21 (Immunoglobulin superfamily member 21) is a GPI-anchored protein that contains two immunoglobulin-like domains (Ig-like 1 and Ig-like 2). The Ig-like 1 domain of IGSF21 is indispensable for synaptogenic activity whereas its Ig-like 2 domain is secondarily responsible for the activity. It has been reported that IGSF21 selectively regulates inhibitory presynaptic differentiation through interacting with presynaptic neurexin2α and plays a crucial role in synaptic inhibition in the brain. (PMID: 28864826)

Publications

Species	Application	Title
mouse	WB	Nat Commun; IgSF21 promotes differentiation of inhibitory synapses via binding to neurexin2α.; Authors - Yuko Tanabe ; KO Validated
human		Front Immunol; Patients' IgLON5 autoantibodies interfere with IgLON5-protein interactions; Authors - Jon Landa
mouse	IHC	Front Immunol; IgLON5 deficiency produces behavioral alterations in a knockout mouse model; Authors - Jon Landa
human,mouse	WB,CoIP	Front Mol Neurosci; Structural and functional characterization of the IgSF21-neurexin2α complex and its related signaling pathways in the regulation of inhibitory synapse organization; Authors - Nicolas Chofflet