IgG Kappa Light Chain Polyclonal antibody

IgG Kappa Light Chain Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 14678-1-AP

Synonyms

IgG Kappa Light Chain, IGK, IGK-, IGKC, immunoglobulin kappa locus

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Validation Data Gallery

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  WB analysis of human plasma using 14678-1-AP

  human plasma (0.3ug) were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14678-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14678-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse spleen using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14678-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14678-1-AP (IgG light chain (Kappa) antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human plasma tissue, human liver tissue, mouse skeletal muscle tissue
• Positive IHC detected in: human spleen tissue, human heart tissue, human placenta tissue, human skin tissue, human testis tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 6 publications below

Product Information

14678-1-AP targets IgG Kappa Light Chain in WB, IHC, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IgG Kappa Light Chain fusion protein Ag6389
• Full Name: immunoglobulin kappa locus
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC030813
• Gene Symbol: IgG Kappa Light Chain
• Gene ID (NCBI): 50802
• RRID: AB_1851235
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the light chain (Kappa) of human IgG.

Publications

Species	Application	Title
	IHC	Nat Commun; A truncating mutation in the autophagy gene UVRAG drives inflammation and tumorigenesis in mice.; Authors - Christine Quach
	IHC	PLoS Genet; Cytosolic O-GlcNAcylation and PNG1 maintain Drosophila gut homeostasis by regulating proliferation and apoptosis.; Authors - Hyun-Jin Na
human	RIP	J Bone Miner Metab; Regulation of circADAMTS6-miR-324-5p-PIK3R3 ceRNA pathway may be a novel mechanism of IL-1β-induced osteoarthritic chondrocytes.; Authors - Lanjuan Shen
mouse	RIP	Mol Med Rep; Long non‑coding RNA XIST promotes cerebral ischemia/reperfusion injury by modulating miR‑27a‑3p/FOXO3 signaling.; Authors - Hua Zhang
mouse	RIP	J Exp Clin Cancer Res; RNA N6-methyladenosine reader IGF2BP2 promotes lymphatic metastasis and epithelial-mesenchymal transition of head and neck squamous carcinoma cells via stabilizing slug mRNA in an m6A-dependent manner.; Authors - Dan Yu
human	IP	Heliyon; HMGA2 overexpression activates IGF2BP2 to stabilize APLP2 via m6A modification and promote pancreatic cancer progression; Authors - Ke Liu