IGHMBP2 Polyclonal antibody

IGHMBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23945-1-AP

Synonyms

ATP dependent helicase IGHMBP2, CATF1, DNA binding protein SMUBP 2, GF 1, Glial factor 1, HCSA, HMN6, IGHMBP2, SMARD1, SMBP2, SMUBP2

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Validation Data Gallery

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  WB analysis using 23945-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23945-1-AP (IGHMBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 23945-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 23945-1-AP (IGHMBP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 23945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23945-1-AP (IGHMBP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 23945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 23945-1-AP (IGHMBP2 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells, human brain tissue, Jurkat cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 23945-1-AP is IGHMBP2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGHMBP2 fusion protein Ag21057
• Full Name: immunoglobulin mu binding protein 2
• Calculated Molecular Weight: 993 aa, 109 kDa
• Observed Molecular Weight: 109 kDa
• GenBank Accession Number: BC105088
• Gene Symbol: IGHMBP2
• Gene ID (NCBI): 3508
• RRID: AB_2879371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGHMBP2 also named as immunoglobulin mu binding protein 2 is a 993 amino-acid protein, which localizes in nuclear and cytoplasm. The molecular weight of IGHMBP2 is 109 kDa. IGHMBP2 belongs to the DNA2/NAM7 helicase family. IGHMBP2 also acts as a transcriptional regulator and is necessary for transcriptional activation of the flounder liver-type antifreeze protein gene. IGHMBP2 exists as a homooligomer and is part of the cytosolic ribonucleoprotein complex.

Publications

Species	Application	Title
human	WB	bioRxiv; IGHMBP2 deletion suppresses translation and activates the integrated stress response; Authors - Jesslyn E Park