human IgM Polyclonal antibody

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• human IgM Polyclonal antibody (11016-1-AP)

human IgM Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11016-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

IGHM, IgM, MU, VH

• 

  WB analysis of human plasma using 11016-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 11016-1-AP (IGHM Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 11016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11016-1-AP (human IgM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 11016-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human tonsillitis using 11016-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11016-1-AP (IGHM antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11016-1-AP targets human IgM in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1459

  Product name: Recombinant human IGHM protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC009851

  Sequence: FGNPRKSKLICQATGFSPRQIQVSWLREGKQVGSGVTTDQVQAEAKESGPTTYKVTSTLTIKESDWLSQSMFTCRVDHRGLTFQQNASSMCVPDQDTAIRVFAIPPSFASIFLTKSTKLTCLVTDLTTYDSVTISWTRQNGEAVKTHTNISESHPNATFSAVGEASICEDDWNSGERFTCTVTHTDLPSPLKQTISRPKGVALHRPDVYLLPPAREQLNLRESATITCLVTGFSPADVFVQWMQRGQPLSPEKYVTSAPMPEPQAPGRYFAHSILTVSEEEWNTGETYTCVVAHEALPNRVTERTVDKSTEGEVSADEEGFENLWATASTFIVLFLLSLFYSTTVTLFKVK

• Full Name: immunoglobulin heavy constant mu
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC009851
• Gene Symbol: IGHM
• Gene ID (NCBI): 3507
• RRID: AB_593462
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This antibody detects the heavy chain of human IgM (~75 kDa).

Publications

What published studies show

The anti-IgM polyclonal antibody (Cat# 11016-1-AP) has 2 citations. They appear in Nature Communications (2025), covering tumor-targeted drug delivery using carbon quantum dots in mouse models, and Journal of Proteomics (2021), addressing tear dynamics and quantitative proteomics in chronic renal failure patients. Both studies utilized this antibody for Western blot analysis.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; Sterically chained amino acid-rich water-soluble carbon quantum dots as a robust tumor-targeted drug delivery platform; Authors - Wenjing Xie
human	WB	J Proteomics; Tear dynamics testing and quantitative proteomics analysis in patients with chronic renal failure.; Authors - Rong-Rong Zong