Human IgA Heavy Chain Polyclonal antibody

Human IgA Heavy Chain Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11449-1-AP

Synonyms

Human IgA, IGHA1, IGHA 2, IGHA 1, Igh2

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Validation Data Gallery

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  WB analysis of human plasma using 11449-1-AP

  human plasma (0.3 ug) were subjected to SDS PAGE followed by western blot with 11449-1-AP (Human IgA Heavy Chain antibody at dilution of 1:1000. (1 Sec exposure) incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11449-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11449-1-AP (IGHA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 11449-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11449-1-AP (Human IgA Heavy Chain antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11449-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11449-1-AP (Human IgA Heavy Chain antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11449-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11449-1-AP (Human IgA Heavy Chain antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 11449-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Human IgA Heavy Chain antibody (11449-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Ramos using 11449-1-AP

  1x10^6 Ramos cells were intracellularly stained with 0.4 ug Human IgA Heavy Chain Polyclonal antibody (11449-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: human plasma tissue, human lung tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue
• Positive FC (Intra) detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:5000-1:20000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

11449-1-AP targets Human IgA Heavy Chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Human IgA Heavy Chain fusion protein Ag2007
• Full Name: immunoglobulin heavy constant alpha 1
• Calculated Molecular Weight: 496 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC016369
• Gene Symbol: Human IgA Heavy Chain
• Gene ID (NCBI): 3493
• RRID: AB_833092
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).

Publications

Species	Application	Title
human	IHC	J Infect; Immune responses and residual SARS-CoV-2 in two critically ill COVID-19 patients before and after lung transplantation.; Authors - Yan Yan
	WB	Cell Immunol; Mesenchymal stem cell transplantation worsens intestinal inflammation and microenvironment in PI3Kγ-knockout mice; Authors - Yi Li
human	WB	Sci Rep; A prospective study revealing the role of an immune-related eRNA, WAKMAR2, in breast cancer; Authors - LinbangWang
human	WB	Sci Rep; Natural antibodies and CRP drive anaphylatoxin production by urate crystals.; Authors - Anne Kathrin Wessig
mouse	WB	Int Immunopharmacol; Altered microbial community structure in PI3Kγ knockout mice with colitis impeding relief of inflammation: Establishment of new indices for intestinal microbial disorder.; Authors - Yi Li
	IHC	Evid Based Complement Alternat Med; Calycosin Improves Intestinal Mucosal Barrier Function after Gastrectomy in Rats through Alleviating Bacterial Translocation, Inflammation, and Oxidative Stress.; Authors - Hui Peng