IGFBP4 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

IGFBP4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18500-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of C6 using 18500-1-AP (same clone as 18500-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 18500-1-AP (same clone as 18500-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 18500-1-AP (IGFBP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18500-1-AP (same clone as 18500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 18500-1-AP (same clone as 18500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 18500-1-AP (same clone as 18500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 18500-1-AP (same clone as 18500-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 18500-1-AP (IGFBP4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

• 

  IF Staining of C6 using 18500-1-AP (same clone as 18500-1-PBS)

  Immunofluorescent analysis of C6 cells using 18500-1-AP (IGFBP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18500-1-PBS in a different storage buffer formulation.

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Product Information

18500-1-PBS targets IGFBP4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGFBP4 fusion protein Ag13347
• Full Name: insulin-like growth factor binding protein 4
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-40 kDa
• GenBank Accession Number: BC016041
• Gene Symbol: IGFBP4
• Gene ID (NCBI): 3487
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IGFBP4, also named as IBP4, is a 258 amino acid protein, which contains one IGFBP N-terminal domain and one thyroglobulin type-1 domain. IGFBP4 is the smallest member of the insulin-like growth factor binding protein family (IGFBP). It is a hepatic protein that plays a role in modulating the activity and bioavailability of IGF-I. The expression of IGFBP4 was found to increase under conditions of hypoxia. IGFBP4 is highly expressed in adipocytes and osteoblasts and is inhibitory of IGFs in vitro. (PMID: 28938423, PMID: 34646401). IGFBP4 is a secreted protein and calculated molecular weight is 28 kDa.