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IGFBP3 Recombinant antibody, PBS Only

IGFBP3 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241723B3

Cat no : 84470-4-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using IGFBP3 Recombinant antibody (84470-4-RR)

    WB analysis of SKOV-3 using 84470-4-RR (same clone as 84470-4-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 84470-4-RR (IGFBP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84470-4-PBS in a different storage buffer formulation.

Product Information

84470-4-PBS targets IGFBP3 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein
Full Name insulin-like growth factor binding protein 3
Calculated Molecular Weight32 kDa
Observed Molecular Weight 32-37 kDa
GenBank Accession NumberNM_000598.5
Gene Symbol IGFBP3
Gene ID (NCBI) 3486
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)