IGFBP3 Polyclonal antibody

IGFBP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30593-1-AP

Synonyms

AI649005, IGFBP 3, Igfbp3, IGgfbp3

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Validation Data Gallery

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  WB analysis using 30593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30593-1-AP (IGFBP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30593-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IGFBP3 antibody (30593-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, mouse colon tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30593-1-AP targets IGFBP3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: insulin-like growth factor binding protein 3
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 31-45 kDa
• GenBank Accession Number: NM_008343
• Gene Symbol: Igfbp3
• Gene ID (NCBI): 16009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)