IGFBP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

IGFBP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

34

Reviews (1)

Cat no : 10189-2-AP

Synonyms

IGF-binding protein 3, IGF binding protein 3, IBP-3, IBP3, IBP 3

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Validation Data Gallery

• 

  WB analysis of mouse colon using 10189-2-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10189-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 10189-2-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10189-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10189-2-AP (IGFBP3 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10189-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10189-2-AP (IGFBP3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10189-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 10189-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IGFBP3 antibody (10189-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: mouse colon tissue, HeLa cells, A2780 cells, HepG2 cells
• Positive IHC detected in: human breast cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 9 publications below
• IF: See 8 publications below

Product Information

10189-2-AP targets IGFBP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGFBP3 fusion protein Ag0242
• Full Name: insulin-like growth factor binding protein 3
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32-37 kDa
• GenBank Accession Number: BC000013
• Gene Symbol: IGFBP3
• Gene ID (NCBI): 3486
• RRID: AB_2123233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447)

Publications

Species	Application	Title
human	IF	Cell; 3D reconstruction of a gastrulating human embryo; Authors - Zhenyu Xiao
human,mouse	WB,IHC,IF	Theranostics; Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p; Authors - Soo Hyun Kang ; KD Validated
		Proc Natl Acad Sci U S A; Disruption of the AMPK-TBC1D1 nexus increases lipogenic gene expression and causes obesity in mice via promoting IGF1 secretion.; Authors - Liang Chen
mouse	WB	Acta Physiol (Oxf); Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1.; Authors - Yingying Zhang
human	WB	Cancer Lett; Choline-induced SLC5A7 impairs colorectal cancer growth by stabilizing p53 protein.; Authors - Yuan Yin
mouse	WB	PLoS Biol; The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis.; Authors - Patrick A Carroll

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Margarita (Verified Customer) (04-19-2023)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: PC3 cells

IGFBP3 Antibody Immunofluorescence validation (1:100 dilution) in PC3 cells (Cat no:10189-2-AP)

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