IGFBP2 Monoclonal antibody

IGFBP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F10D1

Cat no : 66644-1-Ig

Synonyms

BP2, IBP 2, IBP2, IGF binding protein 2, IGF BP53, IGFBP 2, IGFBP2

---

Validation Data Gallery

• 

  WB analysis using 66644-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66644-1-Ig (IGFBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: A549 cells, T-27D cells, MCF-7 cells, PC-3 cells, NCCIT cells, 4T1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66644-1-Ig targets IGFBP2 in WB, ELISA applications and shows reactivity with Human, Mouse, rat samples.

• Tested Reactivity: Human, Mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IGFBP2 fusion protein Ag8421
• Full Name: IGF binding protein 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004312
• Gene Symbol: IGFBP2
• Gene ID (NCBI): 3485
• RRID: AB_2882002
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. The levels of IGFBP2 are elevated during progression of many human cancers.