IGFBP2 Polyclonal antibody

IGFBP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15699-1-AP

Synonyms

IGF binding protein 2, IBP-2, IBP2, IBP 2, BP2

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Validation Data Gallery

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  WB analysis of MCF-7 using 15699-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15699-1-AP (IGFBP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15699-1-AP (IGFBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 15699-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15699-1-AP (IGFBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15699-1-AP

  Immunofluorescent analysis of HeLa cells using 15699-1-AP (IGFBP2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15699-1-AP targets IGFBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGFBP2 fusion protein Ag8327
• Full Name: IGF binding protein 2
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC004312
• Gene Symbol: IGFBP2
• Gene ID (NCBI): 3485
• RRID: AB_10641053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. IGFBP-2 is a 34 kD IGFBP protein with degarded fragments of 24 kD, 19kd and 15kd. The levels of IGFBP2 are elevated during progression of many human cancers. IGFBP2 is expressed in invasive PAc, whereas its expression in HG-PIN is low. It can be as an Immunohistochemical Marker for Prostatic Adenocarcinoma.