IGFBP1 Recombinant antibody

IGFBP1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240263B9

Cat no : 83347-5-RR

Synonyms

IBP-1, IBP1, IBP 1, hIGFBP 1, AFBP

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Validation Data Gallery

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  WB analysis of HepG2 using 83347-5-RR

  HepG2 cells were subjected to SDS PAGE followed by western blot with 83347-5-RR (IGFBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83347-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83347-5-RR against Human IGFBP1 were performed. The affinity constant is 40.6 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83347-5-RR is IGFBP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: IGF binding protein 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: NM_000596.4
• Gene Symbol: IGFBP1
• Gene ID (NCBI): 3484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.