IGFBP1 Polyclonal antibody, PBS Only

• 

  WB analysis using 13981-1-AP (same clone as 13981-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13981-1-AP (IGFBP1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13981-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 13981-1-AP (same clone as 13981-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13981-1-AP (IGFBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13981-1-PBS in a different storage buffer formulation.

IGFBP1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 13981-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

13981-1-PBS targets IGFBP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IGFBP1 fusion protein Ag5026
• Full Name: IGF binding protein 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-35 kDa
• GenBank Accession Number: BC057806
• Gene Symbol: IGFBP1
• Gene ID (NCBI): 3484
• RRID: AB_2233481
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08833
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IGF-binding proteins (IGFBPs) family contains members like IGFBP-1, -2, -3, -4,-5 and-6. IGFBPs prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. IGF-I physically interacts with IGFBP-1 and that IGFBP-1 also binds to an integrin receptor, but the effect of IGFBP-1 on cell migration may be independent of IGF-I and is probably mediated through the alpha 5 beta 1 integrin. Transgenic mice models demonstrated that IGFBPs played important roles in the pathogenesis of obesity and INS resistance. Studies conducted in humans demonstrated the close relation between IGFBPs and the components of the metabolic syndrome.