Rat IGF1 Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP01547-3

  Sandwich ELISA standard curve of MP01547-3, Rat IGF1 Recombinant Matched Antibody Pair - PBS only. 84782-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1976. 84782-1-PBS was HRP conjugated as the detection antibody. Range: 3.91-250 pg/mL

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  Sample test of MP01547-3

  Serum of six mice was measured. The IGF1 concentration of detected samples was determined to be 780.6 pg/mL with a range of 585.0 - 1,089.6 ng/mL.

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  Sample test of MP01547-3

  Rat heart tissue (chopped in 1-2 mm piecesin 30 mL of medium) was cultured for 3 days in RPMI supplemented with 10% fetal calfserum and 50 μM β-mercaptoethanol and stimulated with 2.5 ng/mL LPS. J774.A1 cells (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 20 ng/mL IFN-γ, and 1.0 μg/mL LPS for 3 days. Aliquots of the cell culture supernates were removed, assayed for levels of IGF1, and measured 4,740.2 pg/mL in rat heart cell culture supernatant and 28.04 pg/mL in J774A.1 cell culture supernatant.

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  Sample test of MP01547-3

  Rat lung tissue and mouse heart tissue were rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤ -20 °C. Homogenates were thawed and centrifuged for 5 minutes at 5000 x g. Homogenates were assayed for IGF1 and measured 1.6 ng/mL in rat lung tissue homogenate based on a 0.3 mg/mL homogenate load, and 0.7 ng/mL in mouse heart tissue homogenate based on a 1.4 mg/mL homogenate load.

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  Sample test of MP01547-3

  Serum of six rats was measured. The IGF1 concentration of detected samples was determined to be 1,325.1 pg/mL with a range of 892.6 - 1,922.3 ng/mL.

IGF1 Uni-rAb^TM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84782-6-PBS

Clone No.241890G4

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

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Product Information

84782-6-PBS targets IGF1 as part of a matched antibody pair:

MP01547-3: 84782-6-PBS capture and 84782-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IGF1 fusion protein Eg1976
• Full Name: insulin-like growth factor 1
• Calculated Molecular Weight: 14kd
• GenBank Accession Number: AAA41386
• Gene Symbol: Igf1
• Gene ID (NCBI): 24482
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08025
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.