IGBP1 Monoclonal antibody

IGBP1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D11A8

Cat no : 60256-1-Ig

Synonyms

ALPHA 4, CD79a binding protein 1, IBP1, IGBP1, Protein alpha 4

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Validation Data Gallery

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  WB analysis of K-562 using 60256-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60256-1-Ig (IGBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 60256-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 60256-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 60256-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 60256-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 60256-1-Ig (IGBP1 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human heart tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 60256-1-Ig is IGBP1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IGBP1 fusion protein Ag6782
• Full Name: immunoglobulin (CD79A) binding protein 1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC004137
• Gene Symbol: IGBP1
• Gene ID (NCBI): 3476
• RRID: AB_2881377
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Immunoglobulin-binding protein 1 (IGBP1), also known as ALPHA-4, was originally identified as a signal transduction molecule coprecipitating with MB1 (CD79a) protein of the B-cell antigen receptor (BCR) complex. IGBP1 has been characterized as an essential regulator of the catalytic activity of protein phosphatase 2A (PP2A), which is a ubiquitous phosphatase with broad substrate specificity and diverse cellular functions.