Various lysates were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rabbit brain using 67987-1-Ig
rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 67987-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.
Various lysates were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rabbit brain using 67987-1-Ig
rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67987-1-Ig (IFT81 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse testis using 67987-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 67987-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67987-1-Ig (IFT81 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of hTERT-RPE1 using 67987-1-Ig
Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (67987-1-Ig, Clone: 1D5A3 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IFT81 Monoclonal antibody
Catalog Number
67987-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IF/ICC : 1:1000-1:4000
Applications
WB, IF/ICC, ELISA
Reactivity
human, rat, mouse, rabbit, canine
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
