IFT81 Polyclonal antibody

IFT81 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10604-2-AP

Synonyms

CDV 1, CDV 1R, CDV1, CDV1R, IFT81

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Validation Data Gallery

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  WB analysis of mouse kidney using 10604-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10604-2-AP (IFT81 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10604-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10604-2-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 10604-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10604-2-AP (IFT81 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10604-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10604-2-AP (IFT81 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, HEK-293 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10604-2-AP targets IFT81 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFT81 fusion protein Ag0870
• Full Name: intraflagellar transport 81 homolog (Chlamydomonas)
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC004536
• Gene Symbol: IFT81
• Gene ID (NCBI): 28981
• RRID: AB_2121968
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.

Publications

Species	Application	Title
human	WB	Hum Mutat; Alu-Alu mediated intragenic duplications in IFT81 and MATN3 are associated with skeletal dysplasias.; Authors - Maria Pettersson