IFNGR1 Recombinant antibody

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  WB analysis using 85344-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85344-1-RR (IFNGR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IFNGR1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 85344-1-RR

Clone No.242632C2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IFN-gamma receptor 1, IFN-gamma R1, IFN gamma receptor 1, IFN gamma R1, CD119

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85344-1-RR targets IFNGR1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interferon gamma receptor 1
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 70-90 kDa
• GenBank Accession Number: NM_000416.3
• Gene Symbol: IFNGR1
• Gene ID (NCBI): 3459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P15260-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)