IFNGR1 Polyclonal antibody

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  WB analysis using 31951-1-AP

  EL-4 cells were subjected to SDS PAGE followed by western blot with 31951-1-AP (IFN-gamma R1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of EL-4 using 31951-1-AP

  IP result of anti-IFNGR1 (IP:31951-1-AP, 4ug; Detection:31951-1-AP 1:600) with EL-4 cells lysate 2080 ug.

IFNGR1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 31951-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, ELISA

Synonyms

Ifngr, IFN-gamma-R-alpha, IFN-gamma-R1, IFN-gamma receptor 1, IFN-gamma R1

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Tested Applications

• Positive WB detected in: EL-4 cells
• Positive IP detected in: EL-4 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31951-1-AP targets IFNGR1 in WB, IP, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interferon gamma receptor 1
• Calculated Molecular Weight: 52kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: NM_010511.3
• Gene Symbol: Ifngr1
• Gene ID (NCBI): 15979
• RRID: AB_3670157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P15261
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)