IFNGR1 Polyclonal antibody

IFNGR1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 31951-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, ELISA

Ifngr, IFN-gamma-R-alpha, IFN-gamma-R1, IFN-gamma receptor 1, IFN-gamma R1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inEL-4 cells
Positive IP detected inEL-4 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31951-1-AP targets IFNGR1 in WB, IP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name interferon gamma receptor 1
Calculated Molecular Weight52kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberNM_010511.3
Gene Symbol Ifngr1
Gene ID (NCBI) 15979
RRIDAB_3670157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP15261
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

Protocols

Product Specific Protocols
WB protocol for IFNGR1 antibody 31951-1-APDownload protocol
IP protocol for IFNGR1 antibody 31951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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