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- KD/KO Validated
IFN-gamma R1 Polyclonal antibody
IFN-gamma R1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 10808-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 10 publications below |
IHC | See 4 publications below |
IF | See 2 publications below |
Product Information
10808-1-AP targets IFN-gamma R1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IFN-gamma R1 fusion protein Ag1114 |
Full Name | interferon gamma receptor 1 |
Calculated Molecular Weight | 54 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC005333 |
Gene Symbol | IFNGR1 |
Gene ID (NCBI) | 3459 |
RRID | AB_2121604 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)
Protocols
Product Specific Protocols | |
---|---|
WB protocol for IFN-gamma R1 antibody 10808-1-AP | Download protocol |
IHC protocol for IFN-gamma R1 antibody 10808-1-AP | Download protocol |
IP protocol for IFN-gamma R1 antibody 10808-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Discov Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation. | ||
Nat Commun DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion. | ||
Dev Cell Interferon-γ resistance and immune evasion in glioma develop via Notch-regulated co-evolution of malignant and immune cells. | ||
Oncogene Low doses of IFN-γ maintain self-renewal of leukemia stem cells in acute myeloid leukemia
| ||
Ecotoxicol Environ Saf Silica-induced macrophage pyroptosis propels pulmonary fibrosis through coordinated activation of relaxin and osteoclast differentiation signaling to reprogram fibroblasts | ||
J Neuropathol Exp Neurol Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes. |