IFN-gamma Monoclonal antibody

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  WB analysis of U-937 using 60101-1-Ig

  U-937 cells were subjected to SDS PAGE followed by western blot with 60101-1-Ig (IFNG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IFN-gamma Monoclonal Antibody for WB, ELISA

Cat No. 60101-1-Ig

Clone No.1C10C6

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Synonyms

IFNG, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN gamma, IFG

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Tested Applications

• Positive WB detected in: U-937 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60101-1-Ig targets IFN-gamma in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: IFN-gamma fusion protein Ag8320
• Full Name: IFN gamma
• Calculated Molecular Weight: 166 aa, 19 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC070256
• Gene Symbol: IFNG
• Gene ID (NCBI): 3458
• ENSEMBL Gene ID: ENSG00000111537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Caprylic acid/ammonium sulfate precipitation
• UNIPROT ID: P01579
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFNG is a soluble cytokine that is the only member of the type II class of IFNs. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.