IFNAR1 Recombinant antibody

IFNAR1 Uni-rAb^TM Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230549C8

Cat no : 83002-4-RR

Synonyms

IFN alpha REC, Cytokine receptor class-II member 1, CRF2-1, CRF2 1, AVP

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Validation Data Gallery

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  WB analysis using 83002-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83002-4-RR (IFNAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of Jurkat using 83002-4-RR

  1x10^6 Jurkat cells were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  FC experiment of K562 using 83002-4-RR

  1x10^6 K562 were surface stained with 0.25 ug IFNAR1 Recombinant antibody (83002-4-RR, Clone:230549C8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 83002-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83002-4-RR against Human IFNAR1 were performed. The affinity constant is 2.04 nM.

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, HepG2 cells, HEK-293 cells, A431 cells, Jurkat cells
• Positive FC detected in: Jurkat cells, K562

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83002-4-RR is IFNAR1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: interferon (alpha, beta and omega) receptor 1
• Calculated Molecular Weight: 557 aa, 64 kDa
• Observed Molecular Weight: 80-140 kDa
• GenBank Accession Number: BC021825
• Gene Symbol: IFNAR1
• Gene ID (NCBI): 3454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon alpha and beta receptor subunit 1 (IFNAR1) is a type I membrane protein that associates with IFNAR2 to form the receptor for type I interferons, including interferons-alpha, -beta, and -lambda. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2, and then increase transcription of the IFN-induced genes whose products exert antiviral, immunomodulatory, and antiproliferative effects.