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CoraLite® Plus 488-conjugated IFN Gamma Monoclonal antibody

IFN Gamma Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E10G7

Cat no : CL488-69007

Synonyms

IFG, IFI, IFN gamma, IFN γ, IFNG, IFN-gamma, IFNγ, IFN-γ, Immune interferon, Interferon gamma, interferon, gamma, NeutraKine® IFN Gamma

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using CoraLite® Plus 488-conjugated IFN Gamma Monoclonal (CL488-69007)

    FC experiment of human PBMCs using CL488-69007

    1X10^6 PMA+ionomycin+protein transport inhibitors treated for 6h human PBMCs were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human IFN Gamma (CL488-69007, Clone:1E10G7), and 0.25 ug CoraLite® Plus 647 Anti-Human CD3 (UCHT1) (CL647-65151, Clone: UCHT1), and 0.25 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC (Intra) detected inPMA+ionomycin+protein transport inhibitors for 6h human PBMCs
Positive FC detected inPMA+ionomycin+protein transport inhibitors for 6h human PBMCs

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-69007 targets IFN Gamma in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IFN Gamma fusion protein HZ-1301
Full Name IFN gamma
Gene Symbol IFNG
Gene ID (NCBI) 3458
RRIDAB_2919565
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays crucial roles in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IFN Gamma antibody CL488-69007Download protocol
Standard Protocols
Click here to view our Standard Protocols