IFITM2 Recombinant antibody

IFITM2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240318B6

Cat no : 83455-5-RR

Synonyms

Interferon-induced transmembrane protein 2, IFITM 2, DSPA2c, 240318B6, 18D



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, SGC-7901 cells, MCF-7 cells, A549 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83455-5-RR is IFITM2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen IFITM2 fusion protein Ag3451
Full Name interferon induced transmembrane protein 2 (1-8D)
Calculated Molecular Weight 132 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC009696
Gene Symbol IFITM2
Gene ID (NCBI) 10581
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in a p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also, they serve as important components of the innate immune system to restrict HIV-1 infection.

Protocols

Product Specific Protocols
WB protocol for IFITM2 antibody 83455-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols