CoraLite®555-conjugated IFITM2/3 Monoclonal antibody

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• KD/KO Validated

IFITM2/3 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2E8D12

Cat no : CL555-66081

Synonyms

IFITM3, Interferon-inducible protein 1-8U, Interferon-induced transmembrane protein 3, DSPA2b, 2E8D12

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Validation Data Gallery

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  IF Staining of U2OS using CL555-66081

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using CoraLite®555 IFITM2/3 antibody (CL555-66081, Clone: 2E8D12 ) at dilution of 1:200.

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  FC experiment of HeLa using CL555-66081

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human IFITM2/3 (CL555-66081, Clone:2E8D12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: U2OS cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL555-66081 targets IFITM2/3 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: IFITM2/3 fusion protein Ag17863
• Full Name: interferon induced transmembrane protein 3 (1-8U)
• Observed Molecular Weight: 15-20 kDa
• GenBank Accession Number: BC070243
• Gene Symbol: IFITM3
• Gene ID (NCBI): 10410
• RRID: AB_2919672
• Conjugate: CoraLite®555 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 557 nm / 570 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

IFITM3, also named as interferon-inducible protein 1-8U, belongs to the CD225 family. It is IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM3 is identified as interferon-induced cellular proteins that restrict infections by retroviruses and filoviruses and of influenza virus and flaviviruses, respectively. IFITM3, the most potent antiviral IFITM, was found to inhibit an uncharacterized early infectious event after VSV endocytosis, but before primary transcription of its viral genome. IFITM proteins are viral restriction factors that can inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. They differentially restrict the entry of a broad range of enveloped viruses, and modulate cellular tropism independently of viral receptor expression. This antibody recognizes both IFITM2 and IFITM3.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C