IFIT1 Recombinant antibody, PBS Only

IFIT1 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240132G11

Cat no : 83423-1-PBS

Synonyms

IFIT 1, IFI-56K, IFI56, IFI 56K, G10P1

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Validation Data Gallery

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  WB analysis of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

  IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SGC-7901 using 83423-1-RR (same clone as 83423-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR (same clone as 83423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR (same clone as 83423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83423-1-RR (same clone as 83423-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifit1 Recombinant Antibody (83423-1-RR, Clone:240132G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83423-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83423-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83423-1-RR against Human IFIT1 were performed. The affinity constant is 0.397 nM.

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Product Information

The immunogen of 83423-1-PBS is IFIT1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IFIT1 fusion protein Ag19691
• Full Name: interferon-induced protein with tetratricopeptide repeats 1
• Calculated Molecular Weight: 478 aa, 55 kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: BC007091
• Gene Symbol: IFIT1
• Gene ID (NCBI): 3434
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.