IFIH1-Specific Polyclonal antibody

IFIH1-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16879-1-AP

Synonyms

IFIH1, MDA5, IFIH 1, Helicard, EC:3.6.4.13

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Validation Data Gallery

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  WB analysis using 16879-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16879-1-AP (IFIH1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 16879-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16879-1-AP (IFIH1-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16879-1-AP targets IFIH1-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: interferon induced with helicase C domain 1
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 70 kDa, 90 kDa
• GenBank Accession Number: NM_022168
• Gene Symbol: IFIH1
• Gene ID (NCBI): 64135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501). This antibody is specific to IFIH1.