Various lysates were subjected to SDS PAGE followed by western blot with 83424-1-RR (IFI44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 83424-1-RR (IFI44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
FC experiment of A549 using 83424-1-RR
1x10^6 A549 cells were intracellularly stained with 0.25 ug IFI44 Recombinant antibody (83424-1-RR, Clone:240022F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
1x10^6 A549 cells were intracellularly stained with 0.25 ug IFI44 Recombinant antibody (83424-1-RR, Clone:240022F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
Affinity and Kinetic Characterization of 83424-1-RR
Biolayer interferometry (BLl) kinetic assays of 83424-1-RR against Human IFI44 were performed. The affinity constant is 5.57 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Various lysates were subjected to SDS PAGE followed by western blot with 83424-1-RR (IFI44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
FC (INTRA) Figures
FC experiment of A549 using 83424-1-RR
1x10^6 A549 cells were intracellularly stained with 0.25 ug IFI44 Recombinant antibody (83424-1-RR, Clone:240022F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
AFFINITY Figures
Affinity and Kinetic Characterization of 83424-1-RR
Biolayer interferometry (BLl) kinetic assays of 83424-1-RR against Human IFI44 were performed. The affinity constant is 5.57 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IFI44 Recombinant antibody
Catalog Number
83424-1-RR
Citations
-
Dilutions
WB : 1:1000-1:8000 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
WB, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
