IFI44 Polyclonal antibody

IFI44 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 27233-1-AP

Synonyms

IFI44, interferon induced protein 44, MTAP44, p44

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Validation Data Gallery

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  WB analysis of mouse kidney using 27233-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27233-1-AP (IFI44 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 27233-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27233-1-AP (IFI44 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 27233-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27233-1-AP (IFI44 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 3 publications below

Product Information

27233-1-AP targets IFI44 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFI44 fusion protein Ag26121
• Full Name: interferon-induced protein 44
• Calculated Molecular Weight: 444 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC022870
• Gene Symbol: IFI44
• Gene ID (NCBI): 10561
• RRID: AB_2880813
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Cell Dev Biol; Integrative Analysis of DNA Methylation and Transcriptome Identifies a Predictive Epigenetic Signature Associated With Immune Infiltration in Gliomas.; Authors - Jianlei Zhang
human	IHC	Front Oncol; Interferon-Induced Protein 44 Correlated With Immune Infiltration Serves as a Potential Prognostic Indicator in Head and Neck Squamous Cell Carcinoma.; Authors - Hua Pan
human	WB	Pathol Res Pract; Long noncoding RNA GAS5 inhibits proliferation and metastasis in papillary thyroid carcinoma through the IFN/STAT1 signaling pathway.; Authors - Yongcan Xu
	WB	BMC Genomics; Annexin A2 (ANXA2) regulates the transcription and alternative splicing of inflammatory genes in renal tubular epithelial cells.; Authors - Jing Chen
human	IHC	Oxid Med Cell Longev; Systematic Analysis of Molecular Subtypes Based on the Expression Profile of Immune-Related Genes in Pancreatic Cancer; Authors - Mujing Ke