IFI30 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFI30 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11597-1-AP

Synonyms

IP30, IFI 30, GILT, Gamma-interferon-inducible protein IP-30, Gamma-interferon-inducible lysosomal thiol reductase

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Validation Data Gallery

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  WB analysis of COLO 320 using 11597-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11597-1-AP (IFI30 Antibody) at dilution of 1:6000 incubated at 4 degree celsius over night.

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  IP experiment of COLO 320 using 11597-1-AP

  IP result of anti-IFI30 (IP:11597-1-AP, 4ug; Detection:11597-1-AP 1:3000) with COLO 320 cells lysate 2400 ug.

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  IHC staining of human liver cancer using 11597-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11597-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11597-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11597-1-AP (IFI30 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: COLO 320 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

11597-1-AP targets IFI30 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFI30 fusion protein Ag2178
• Full Name: interferon, gamma-inducible protein 30
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC021136
• Gene Symbol: IFI30
• Gene ID (NCBI): 10437
• RRID: AB_2121789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFI30(interferon, gamma-inducible protein 30) is also named as IP30, GILT, IFI-30, MGC32056, IFI30 and belongs to the GILT family. It can facilitate the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. IFI30 is inducibly up-regulated by IFN-g, as are MHC class II molecules, the associated invariant chain, and HLA-DM, which catalyses peptide loading in MIICs and the 35 kDa species is the IFI30 proform, and the 30-kDa species is the mature form(PMID:10639150). It can exsit as a dimer(PMID:17142755).

Publications

Species	Application	Title
human	WB,IHC	Int J Med Sci; IFI30 expression predicts patient prognosis in breast cancer and dictates breast cancer cells proliferation via regulating autophagy.; Authors - Yan Fan ; KD Validated
human	WB	Phytomedicine; Nobiletin inhibits de novo FA synthesis to alleviate gastric cancer progression by regulating endoplasmic reticulum stress; Authors - Menglin Chen
human	WB	Aging (Albany NY); Identification and validation of a novel signature based on macrophage marker genes for predicting prognosis and drug response in kidney renal clear cell carcinoma by integrated analysis of single cell and bulk RNA sequencing; Authors - Xiaoxu Chen